Hyaluronic Acid binding protein 2 is a novel regulator of vascular integrity.

ABSTRACT:

Objective

The disruption of the endothelial cell barrier is a critical feature of inflammation and an important contributing factor to acute lung injury (ALI), an inflammatory condition that is a major cause of morbidity and mortality in critically ill patients. We evaluated the role of the extracellular serine protease, hyaluronic acid binding protein 2 (HABP2), in vascular barrier regulation.

Methods and results

By using immunoblot and immunohistochemical analysis, we observed that lipopolysaccharide (LPS) induces HABP2 expression in murine lung endothelium in vivo and in human pulmonary microvascular endothelial cells (ECs) in vitro. High-molecular-weight hyaluronan (HMW-HA, approximately 1x10(6) Da) decreased HABP2 protein expression in human pulmonary microvascular ECs

SUBMITTER: Mambetsariev N 

PROVIDER: S-EPMC2825278 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature