Functional analysis of Kv1.2 and paddle chimera Kv channels in planar lipid bilayers.

ABSTRACT: Voltage-dependent K(+) (Kv) channels play key roles in shaping electrical signaling in both excitable and nonexcitable cells. These channels open and close in response to the voltage changes across the cell membrane. Many studies have been carried out in order to understand the voltage-sensing mechanism. Our laboratory recently determined the atomic structures of a mammalian Kv channel Kv1.2 and a mutant of Kv1.2 named the 'paddle chimera' channel, in which the voltage sensor paddle was transferred from Kv2.1 to Kv1.2. These two structures provide atomic descriptions of voltage-dependent channels with unprecedented clarity. Until now, the functional integrity of these two channels biosynthesized in yeast cells has not been assessed. Here, we report the electrophysiological and pharmacologi

SUBMITTER: Tao X 

PROVIDER: S-EPMC2825521 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature