Selection of mutations to detect multidrug-resistant Mycobacterium tuberculosis strains in Shanghai, China.

ABSTRACT: Novel tools are urgently needed for the rapid, reliable detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of Mycobacterium tuberculosis. To develop such tools, we need information about the frequency and distribution of the mycobacterial mutations and genotypes that are associated with phenotypic drug resistance. In a population-based study, we sequenced specific genes of M. tuberculosis that were associated with resistance to rifampin and isoniazid in 242 phenotypically MDR isolates and 50 phenotypically pan-susceptible isolates from tuberculosis (TB) cases in Shanghai, China. We estimated the sensitivity and specificity of the mutations, using the results of conventional, culture-based phenotypic drug susceptibility testing as the standard. We detected m

SUBMITTER: Luo T 

PROVIDER: S-EPMC2826000 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature