Comparative proteomic analysis of normal and tumor stromal cells by tissue on chip based mass spectrometry (toc-MS).

ABSTRACT: In carcinoma tissues, genetic and metabolic changes not only occur at the tumor cell level, but also in the surrounding stroma. This carcinoma-reactive stromal tissue is heterogeneous and consists e.g. of non-epithelial cells such as fibroblasts or fibrocytes, inflammatory cells and vasculature-related cells, which promote carcinoma growth and progression of carcinomas. Nevertheless, there is just little knowledge about the proteomic changes from normal connective tissue to tumor stroma. In the present study, we acquired and analysed specific protein patterns of small stromal sections surrounding head and neck cell complexes in comparison to normal subepithelial connective tissue. To gain defined stromal areas we used laser-based tissue microdissection. Because these stromal areas are limi

SUBMITTER: Escher N 

PROVIDER: S-EPMC2826342 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature