Ethanol augments PDGF-induced NADPH oxidase activity and proliferation in rat pancreatic stellate cells.

ABSTRACT:

Background/aims

Activated stellate cells are considered the principal mediators of chronic alcoholic pancreatitis/fibrosis. However the mechanisms of alcohol action on pancreatic stellate cells (PaSCs) are poorly understood. The aims of this study were to determine the presence and role of the NADPH oxidase system in mediating alcohol effects on PaSCs with specific emphasis on proliferation.

Methods

PaSC NADPH oxidase components mRNA and protein were determined by RT-PCR and Western blot. The NADPH oxidase activity was measured by detecting the production of reactive oxygen species using lucigenin-derived chemiluminescence assay. PaSC DNA synthesis, a measure of proliferation, was performed by determining the [3H] thymidine incorporation into DNA.

Results

mRNA for NAD

SUBMITTER: Hu R 

PROVIDER: S-EPMC2826430 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature