A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.

ABSTRACT: Structure determination of a novel macromolecular complex via single-particle electron microscopy depends upon overcoming the challenge of establishing a reliable 3-D reconstruction using only 2-D images. There are a variety of strategies that deal with this issue, but not all of them are readily accessible and straightforward to use. We have developed a "toolbox" of ab initio reconstruction techniques that provide several options for calculating 3-D volumes in an easily managed and tightly controlled work-flow that adheres to standard conventions and formats. This toolbox is designed to streamline the reconstruction process by removing the necessity for bookkeeping, while facilitating transparent data transfer between different software packages. It currently includes procedures for calcu

SUBMITTER: Voss NR 

PROVIDER: S-EPMC2826578 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature