Metal transcription factor-1 regulation via MREs in the transcribed regions of selenoprotein H and other metal-responsive genes.

ABSTRACT: Selenoprotein H is a redox-sensing DNA binding protein that upregulates genes involved in antioxidant responses. Given the known links between oxidative stress and heavy metals, we investigated the potential for regulation of selenoprotein H by metals. In silico analysis of the selenoprotein H genes from nine species reveals multiple predicted metal response elements (MREs). To validate MRE function, we investigated the effects of zinc or cadmium addition and metal-responsive transcription factor 1 (MTF-1) knockout on selenoprotein H mRNA levels. Chromatin immunoprecipitation was used to directly assess physical binding of the transcription factor to MREs in the human and mouse selenoprotein H genes. The results reported herein show that selenoprotein H is a newly identified target for MTF

SUBMITTER: Stoytcheva ZR 

PROVIDER: S-EPMC2826586 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature