Proteomics of Thlaspi caerulescens accessions and an inter-accession cross segregating for zinc accumulation.

ABSTRACT: Metal hyperaccumulator plants have previously been characterized by transcriptomics, but reports on other profiling techniques are scarce. Protein profiles of Thlaspi caerulescens accessions La Calamine (LC) and Lellingen (LE) and lines derived from an LCxLE cross were examined here to determine the co-segregation of protein expression with the level of zinc (Zn) hyperaccumulation. Although hydrophobic proteins such as membrane transporters are not disclosed, this approach has the potential to reveal other proteins important for the Zn hyperaccumulation trait. Plants were exposed to metals. Proteins were separated using two-dimensional electrophoresis and those showing differences among accessions, lines or metal exposures were subjected to mass-spectrometric analysis for identification. C

SUBMITTER: Tuomainen M 

PROVIDER: S-EPMC2826651 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature