Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis.

ABSTRACT:

Background

Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential growth on glucose, cells of H. polymorpha typically contain a single, small peroxisome that is redundant for growth while on methanol multiple, enlarged peroxisomes are present. These organelles are crucial to support growth on methanol, as they contain key enzymes of methanol metabolism.In this study, changes in the transcriptional profiles during adaptation of H. polymorpha cells from glucose- to methanol-containing media were investigated using

SUBMITTER: van Zutphen T 

PROVIDER: S-EPMC2827406 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature