Expression of the dermatomyositis autoantigen Mi-2 in regenerating muscle.

ABSTRACT:

Objective

Autoantibodies against the chromatin remodeler Mi-2 are found in a distinct subset of patients with dermatomyositis (DM). Previous quantitative immunoblotting experiments demonstrated that Mi-2 protein levels are up-regulated in DM muscle. This study was undertaken to define the population of cells expressing high levels of Mi-2 in DM muscle and to explore the regulation and functional role of Mi-2 during muscle regeneration.

Methods

The expression of Mi-2 was analyzed by immunofluorescence microscopy in human muscle biopsy specimens. In an experimental mouse model, cardiotoxin was used to induce muscle injury and repair, and expression of Mi-2 during muscle regeneration was studied in this model by immunofluorescence and immunoblotting analyses. In addition, a cel

SUBMITTER: Mammen AL 

PROVIDER: S-EPMC2828900 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature