Nuclear translocation of beta-actin is involved in transcriptional regulation during macrophage differentiation of HL-60 cells.

ABSTRACT: Studies have shown that nuclear translocation of actin occurs under certain conditions of cellular stress; however, the functional significance of actin import remains unclear. Here, we demonstrate that during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of HL-60 cells toward macrophages, beta-actin translocates from the cytoplasm to the nucleus and that this process is dramatically inhibited by pretreatment with p38 mitogen-activated protein kinase inhibitors. Using chromatin immunoprecipitation-on-chip assays, the genome-wide maps of beta-actin binding to gene promoters in response to PMA treatment is analyzed in HL-60 cells. A gene ontology-based analysis shows that the identified genes belong to a broad spectrum of functional categories such as cell growth and diff

SUBMITTER: Xu YZ 

PROVIDER: S-EPMC2828967 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature