Mutation discovery in the mouse using genetically guided array capture and resequencing.

ABSTRACT: Forward genetics (phenotype-driven approaches) remain the primary source for allelic variants in the mouse. Unfortunately, the gap between observable phenotype and causative genotype limits the widespread use of spontaneous and induced mouse mutants. As alternatives to traditional positional cloning and mutation detection approaches, sequence capture and next-generation sequencing technologies can be used to rapidly sequence subsets of the genome. Application of these technologies to mutation detection efforts in the mouse has the potential to significantly reduce the time and resources required for mutation identification by abrogating the need for high-resolution genetic mapping, long-range PCR, and sequencing of individual PCR amplimers. As proof of principle, we used array-based sequen

SUBMITTER: D'Ascenzo M 

PROVIDER: S-EPMC2829192 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature