Whole-genome sequencing of a laboratory-evolved yeast strain.

ABSTRACT:

Background

Experimental evolution of microbial populations provides a unique opportunity to study evolutionary adaptation in response to controlled selective pressures. However, until recently it has been difficult to identify the precise genetic changes underlying adaptation at a genome-wide scale. New DNA sequencing technologies now allow the genome of parental and evolved strains of microorganisms to be rapidly determined.

Results

We sequenced >93.5% of the genome of a laboratory-evolved strain of the yeast Saccharomyces cerevisiae and its ancestor at >28x depth. Both single nucleotide polymorphisms and copy number amplifications were found, with specific gains over array-based methodologies previously used to analyze these genomes. Applying a segmentation algorithm to qu

SUBMITTER: Araya CL 

PROVIDER: S-EPMC2829512 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature