Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death.

ABSTRACT:

Background

In situ hybridization is a general molecular method typically used for the localization of mRNA transcripts in plants. The method provides a valuable tool to unravel the connection between gene expression and anatomy, especially in species such as pines which show large genome size and shortage of sequence information.

Results

In the present study, expression of the catalase gene (CAT) related to the scavenging of reactive oxygen species (ROS) and the polyamine metabolism related genes, diamine oxidase (DAO) and arginine decarboxylase (ADC), were localized in developing Scots pine (Pinus sylvestris L.) seeds. In addition to specific signals from target mRNAs, the probes continually hybridized non-specifically in the embryo surrounding region (ESR) of the megagamet

SUBMITTER: Vuosku J 

PROVIDER: S-EPMC2829549 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature