Transcription factors Elk-1 and SRF are engaged in IL1-dependent regulation of ZC3H12A expression.

ABSTRACT:

Background

MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored.

Results

Here we report that the proinflammatory cytokine IL-1beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1beta. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcripti

SUBMITTER: Kasza A 

PROVIDER: S-EPMC2829564 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature