Broad spectrum microarray for fingerprint-based bacterial species identification.

ABSTRACT:

Background

Microarrays are powerful tools for DNA-based molecular diagnostics and identification of pathogens. Most target a limited range of organisms and are based on only one or a very few genes for specific identification. Such microarrays are limited to organisms for which specific probes are available, and often have difficulty discriminating closely related taxa. We have developed an alternative broad-spectrum microarray that employs hybridisation fingerprints generated by high-density anonymous markers distributed over the entire genome for identification based on comparison to a reference database.

Results

A high-density microarray carrying 95,000 unique 13-mer probes was designed. Optimized methods were developed to deliver reproducible hybridisation patterns that

SUBMITTER: Pasquer F 

PROVIDER: S-EPMC2830168 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature