Arsenite suppression of involucrin transcription through AP1 promoter sites in cultured human keratinocytes.

ABSTRACT: While preserving keratinocyte proliferative ability, arsenite suppresses cellular differentiation markers by preventing utilization of AP1 transcriptional response elements. In present experiments, arsenite had a dramatic effect in electrophoretic mobility supershift analysis of proteins binding to an involucrin promoter AP1 response element. Without arsenite treatment, binding of JunB and Fra1 was readily detected in nuclear extracts from preconfluent cultures and was not detected a week after confluence, while c-Fos was detected only after confluence. By contrast, band shift of nuclear extracts from arsenite treated cultures showed only JunB and Fra1 binding in postconfluent as well as preconfluent cultures. Immunoblotting of cell extracts showed that arsenite treatment prevented the los

SUBMITTER: Sinitsyna NN 

PROVIDER: S-EPMC2830317 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature