Sensitive plasma protein analysis by microparticle-based proximity ligation assays.

ABSTRACT: Detection of proteins released in the bloodstream from tissues damaged by disease can promote early detection of pathological conditions, differential diagnostics, and follow-up of therapy. Despite these prospects and a plethora of candidate biomarkers, efforts in recent years to establish new protein diagnostic assays have met with limited success. One important limiting factor has been the challenge of detecting proteins present at trace levels in complex bodily fluids. To achieve robust, sensitive, and specific detection, we have developed a microparticle-based solid-phase proximity ligation assay, dependent on simultaneous recognition of target proteins by three antibody molecules for added specificity. After capture on a microparticle, solid-phase pairs of proximity probes are added f

SUBMITTER: Darmanis S 

PROVIDER: S-EPMC2830843 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature