In-depth sequencing of the siRNAs associated with peach latent mosaic viroid infection.

ABSTRACT:

Background

It has been observed that following viroid infection, there is an accumulation of viroid-derived siRNAs in infected plants. Some experimental results suggest that these small RNAs may be produced by the plant defense system to protect it from infection, indicating that viroids can elicit the RNA-silencing pathways. The objective of this study is to identify in the peach latent mosaic viroid (PLMVd), a model RNA genome, the regions that are most susceptible to RNA interference machinery.

Results

The RNA isolated from an infected tree have been used to sequence in parallel viroid species and small non-coding RNA species. Specifically, PLMVd RNAs were amplified, cloned and sequenced according to a conventional approach, while small non-coding RNAs were determined by

SUBMITTER: Bolduc F 

PROVIDER: S-EPMC2830927 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature