Analysis of differential DNA damage in the mitochondrial genome employing a semi-long run real-time PCR approach.

ABSTRACT: The maintenance of the mitochondrial genomic integrity is a prerequisite for proper mitochondrial function. Due to the high concentration of reactive oxygen species (ROS) generated by the oxidative phosphorylation pathway, the mitochondrial genome is highly exposed to oxidative stress leading to mitochondrial DNA injury. Accordingly, mitochondrial DNA damage was shown to be associated with ageing as well as with numerous human diseases including neurodegenerative disorders and cancer. To date, several methods have been described to detect damaged mitochondrial DNA, but those techniques are semi-quantitative and often require high amounts of genomic input DNA. We developed a rapid and quantitative method to evaluate the relative levels of damage in mitochondrial DNA by using the real time-P

SUBMITTER: Rothfuss O 

PROVIDER: S-EPMC2831309 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature