Quantitation of PAX6 and PAX6(5a) transcript levels in adult human lens, cornea, and monkey retina.

ABSTRACT:

Purpose

PAX6 is a critical regulator of the developing lens, other ocular tissues, central nervous system, and pancreas. There are two alternatively spliced forms of the protein, PAX6 and PAX6(5a), that may have different regulatory functions. This study was designed to determine the amounts of PAX6 and PAX6(5a) transcripts present in adult human lens epithelium and fibers, human cornea and monkey retina.

Methods

PAX6 and PAX6(5a) transcript levels were monitored in microdissected lens epithelia, lens fibers, whole lens, cornea, and retina by competitive RT-PCR. The levels of TBP/TFIID were examined in adult human lens epithelium and fibers as control.

Results

PAX6 and PAX6(5a) were expressed at equal levels in lens epithelium and fibers. Ninety-five times more PAX6 t

SUBMITTER: Zhang W 

PROVIDER: S-EPMC2831401 | biostudies-literature | 2001 Jan

REPOSITORIES: biostudies-literature