Decreased expression of ribosomal proteins in human age-related cataract.

ABSTRACT:

Purpose

To identify lens epithelial genes with altered expression levels in age-related human cataract.

Methods

Epithelia from age-related cataracts and from normal lenses were microdissected and RNA was extracted. RNAs were compared for gene expression differences by RT-PCR differential display. Transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The expression levels of identified clones were confirmed by semiquantitative RT-PCR with three separately isolated RNA preparations. Specific primers were designed and used to examine the mRNA levels of other genes important in protein synthesis.

Results

Numerous transcripts exhibited altered levels of gene expression. One transcript exhibiting a decreased level of expression i

SUBMITTER: Zhang W 

PROVIDER: S-EPMC2831404 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature