Increased susceptibility of Huh7 cells to HCV replication does not require mutations in RIG-I.

ABSTRACT:

Background

The cytosolic retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor that senses HCV double-stranded RNA and triggers type I interferon pathways. The clone Huh7.5 of human hepatoma Huh7 cells contains a mutation in RIG-I that is believed to be responsible for the improved replication of HCV in these cells relative to the parental strain. We hypothesized that, in addition to RIG-I, other determinant(s) outside the RIG-I coding sequence are involved in limiting HCV replication in cell culture. To test our hypothesis, we analyzed Huh7 cell clones that support the efficient replication of HCV and analyzed the RIG-I gene.

Results

One clone, termed Huh7D, was more permissive for HCV replication and more efficient for HCV-neomycin and HCV-hygromycin bas

SUBMITTER: Feigelstock DA 

PROVIDER: S-EPMC2831881 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature