Scyl1 regulates Golgi morphology.

ABSTRACT:

Background

Membrane trafficking is a defining feature of eukaryotic cells, and is essential for the maintenance of organelle homeostasis and identity. We previously identified Scy1-like 1 (Scyl1), a member of the Scy1-like family of catalytically inactive protein kinases, as a high-affinity binding partner of COPI coats. COPI-coated vesicles control Golgi to endoplasmic reticulum trafficking and we observed that disruption of Scyl1 function leads to a decrease in trafficking of the KDEL receptor via the COPI pathway. We reasoned that if Scyl1 plays a major role in COPI trafficking its disruption could influence Golgi homeostasis.

Methodology/principal findings

We performed Scyl1 knock down in cultured cells using previously established methods and observed an alteration in G

SUBMITTER: Burman JL 

PROVIDER: S-EPMC2832016 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature