Preparation of genomic DNA from a single species of uncultured magnetotactic bacterium by multiple-displacement amplification.

ABSTRACT: Magnetotactic bacteria comprise a phylogenetically diverse group that is capable of synthesizing intracellular magnetic particles. Although various morphotypes of magnetotactic bacteria have been observed in the environment, bacterial strains available in pure culture are currently limited to a few genera due to difficulties in their enrichment and cultivation. In order to obtain genetic information from uncultured magnetotactic bacteria, a genome preparation method that involves magnetic separation of cells, flow cytometry, and multiple displacement amplification (MDA) using phi29 polymerase was used in this study. The conditions for the MDA reaction using samples containing 1 to 100 cells were evaluated using a pure-culture magnetotactic bacterium, "Magnetospirillum magneticum AMB-1," wh

SUBMITTER: Arakaki A 

PROVIDER: S-EPMC2832384 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature