Sequence-based identification of filamentous basidiomycetous fungi from clinical specimens: a cautionary note.

ABSTRACT: The species-level identification of sterile and/or arthroconidium-forming filamentous fungi presumed to be basidiomycetes based upon morphological or physiological features alone is usually not possible due to the limited amount of hyphal differentiation. Therefore, a reliable molecular approach capable of the unambiguous identification of clinical isolates is needed. One hundred sixty-eight presumptive basidiomycetes were screened by sequence analysis of the internal transcribed spacer (ITS) and D1/D2 ribosomal DNA regions in an effort to obtain a species identification. Through the use of this approach, identification of a basidiomycetous fungus to the species level was obtained for 167/168 of the isolates. However, comparison of the BLAST results for each isolate for both regions reveal

SUBMITTER: Romanelli AM 

PROVIDER: S-EPMC2832452 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature