Amplitude histogram-based method of analysis of patch clamp recordings that involve extreme changes in channel activity levels.

ABSTRACT: Many ion channels show low basal activity, which is increased hundreds-fold by the relevant gating factor. A classical example is the activation G-protein-activated K(+) channels (GIRK) by Gbetagamma subunit dimer. The extent of activation (relative to basal current), R(a), is an important physiological parameter, usually readily estimated from whole cell recordings. However, calculation of R(a) often becomes non-trivial in multi-channel patches because of extreme changes in activity upon activation, from a seemingly single-channel pattern to a macroscopic one. In such cases, calculation of the net current flowing through the channels in the patch, I, before and after activation may require different methods of analysis. To address this problem, we utilized neuronal GIRK channels activated

SUBMITTER: Yakubovich D 

PROVIDER: S-EPMC2832599 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature