The APC tumor suppressor inhibits DNA replication by directly binding to DNA via its carboxyl terminus.

ABSTRACT:

Background & aims

The APC tumor suppressor is well known for its ability to regulate Wnt signaling through mediation of beta-catenin levels in the cell. Transient over expression of the tumor suppressor gene APC in colon cancer cells prevents entry into S phase of the cell cycle, a phenotype only partially restored by cotransfection of a transcriptionally active form of beta-catenin. In an attempt to define its transcription-independent tumor suppressor functions, we tested whether APC directly affects DNA replication.

Methods

A transcriptionally quiescent in vitro DNA replication system, the polymerase chain reaction, DNA binding assays, and transient transfections in colon cancer cell lines were used to determine the effects of APC on DNA replication and the mechanism by w

SUBMITTER: Qian J 

PROVIDER: S-EPMC2832605 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature