MEPE/OF45 as a new target for sensitizing human tumour cells to DNA damage inducers.

ABSTRACT:

Background

We recently identified matrix extracellular phosphoglycoprotein/osteoblast factor 45 (MEPE/OF45) as a new cofactor of CHK1 in rat cells. The aim of this study was to determine the role of human MEPE/OF45 (hMEPE/OF45 has approximately 50% homology with rat MEPE/OF45 (rMEPE/OF45)) in affecting the sensitivity of human tumour cells to DNA damage.

Methods

hMEPE/OF45 expression in different human tumour cell lines and its relevance to the resistance of cell lines to DNA damage inducers such as ionising radiation (IR) or camptothecin (CPT) were assessed. Cells lines stably expressing wild-type MEPE/OF45 or mutant MEPE/OF45 (with the CHK1 interactive key domain (amino acids 488-507) deleted) were established. Cell survival, G(2) accumulation, CHK1 half-life and the CHK1

SUBMITTER: Zhang P 

PROVIDER: S-EPMC2833259 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature