Mass spectral analysis of neuropeptide expression and distribution in the nervous system of the lobster Homarus americanus.

ABSTRACT: The lobster Homarus americanus has long served as an important animal model for electrophysiological and behavioral studies. Using this model, we performed a comprehensive investigation of the neuropeptide expression and their localization in the nervous system, which provides useful insights for further understanding of their biological functions. Using nanoLC ESI Q-TOF MS/MS and three types of MALDI instruments, we analyzed the neuropeptide complements in a major neuroendocrine structure, pericardial organ. A total of 57 putative neuropeptides were identified and 18 of them were de novo sequenced. Using direct tissue/extract analysis and bioinformatics software SpecPlot, we charted the global distribution of neuropeptides throughout the nervous system in H. americanus. Furthermore, we al

SUBMITTER: Chen R 

PROVIDER: S-EPMC2833335 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature