Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.

ABSTRACT:

Background

Efficient expression systems exist for antibody (Ab) molecules, which allow for characterization of large numbers of individual Ab variants. In contrast, such expression systems have been lacking for soluble T cell receptors (TCRs). Attempts to generate bacterial systems have generally resulted in low yields and material which is prone to aggregation and proteolysis. Here we present an optimized periplasmic bacterial expression system for soluble single chain (sc) TCRs.

Results

The effect of 1) over-expression of the periplasmic chaperon FkpA, 2) culture conditions and 3) molecular design was investigated. Elevated levels of FkpA allowed periplasmic soluble scTCR expression, presumably by preventing premature aggregation and inclusion body formation. Periplasmic e

SUBMITTER: Gunnarsen KS 

PROVIDER: S-EPMC2834602 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature