High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak.

ABSTRACT:

Background

A large, multi-province outbreak of listeriosis associated with ready-to-eat meat products contaminated with Listeria monocytogenes serotype 1/2a occurred in Canada in 2008. Subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (PFGE) revealed two similar but distinct AscI PFGE patterns. High-throughput pyrosequencing of two L. monocytogenes isolates was used to rapidly provide the genome sequence of the primary outbreak strain and to investigate the extent of genetic diversity associated with a change of a single restriction enzyme fragment during PFGE.

Results

The chromosomes were collinear, but differences included 28 single nucleotide polymorphisms (SNPs) and three indels, including a 33 kbp prophage that accounted for the observed d

SUBMITTER: Gilmour MW 

PROVIDER: S-EPMC2834635 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature