Baculovirus immediately early 1, a mediator for homologous regions enhancer function in trans.

ABSTRACT:

Background

Enhancers are DNA sequences that serve as binding sites for regulatory proteins, and stimulate transcriptional activity independent of their positions and orientations with respect to the transcriptional initiation site. Previous studies considered that baculovirus homologous regions (hrs) function as enhancers in cis. In our study, a plasmid containing homologous region 3 (hr3) enhancer from Bombyx mori nucleopolyhedrovirus (BmNPV) failed to enhance transcription of promoter in other plasmid in co-transfection assays, but strong stimulation occurred when cells were infected by BmNPV.

Results

The cotransfection results of each BmNPV genomic library plasmid, hr3 plasmid and reporter plasmid showed that there were eight library plasmids stimulated the luciferase gen

SUBMITTER: Lin X 

PROVIDER: S-EPMC2834656 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature