Quantitative analysis of age specific variation in the abundance of human female parotid salivary proteins.

ABSTRACT: Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of pooled saliva collected from the parotid glands of healthy female subjects, divided into two age groups 1 and 2 (20-30 and 55-65 years old, respectively). Hydrophobic charge interaction chromatography was used to separate high- from low-abundance proteins prior to characterization of the parotid saliva using multidimensional protein identification technology (MudPIT). Collectively, 532 proteins were identified in the two age groups. Of these proteins, 266 were identified exclusivel

SUBMITTER: Ambatipudi KS 

PROVIDER: S-EPMC2834885 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature