Isolation, characterization and heterologous expression of a novel chitosanase from Janthinobacterium sp. strain 4239.

ABSTRACT:

Background

Chitosanases (EC 3.2.1.132) hydrolyze the polysaccharide chitosan, which is composed of partially acetylated beta-(1,4)-linked glucosamine residues. In nature, chitosanases are produced by a number of Gram-positive and Gram-negative bacteria, as well as by fungi, probably with the primary role of degrading chitosan from fungal and yeast cell walls for carbon metabolism. Chitosanases may also be utilized in eukaryotic cell manipulation for intracellular delivery of molecules formulated with chitosan as well as for transformation of filamentous fungi by temporal modification of the cell wall structures.However, the chitosanases used so far in transformation and transfection experiments show optimal activity at high temperature, which is incompatible with most transfection

SUBMITTER: Johnsen MG 

PROVIDER: S-EPMC2835661 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature