Proteomic profiling of the NCI-60 cancer cell lines using new high-density reverse-phase lysate microarrays.

ABSTRACT: Because most potential molecular markers and targets are proteins, proteomic profiling is expected to yield more direct answers to functional and pharmacological questions than does transcriptional profiling. To aid in such studies, we have developed a protocol for making reverse-phase protein lysate microarrays with larger numbers of spots than previously feasible. Our first application of these arrays was to profiling of the 60 human cancer cell lines (NCI-60) used by the National Cancer Institute to screen compounds for anticancer activity. Each glass slide microarray included 648 lysate spots representing the NCI-60 cell lines plus controls, each at 10 two-fold serial dilutions to provide a wide dynamic range. Mouse monoclonal antibodies and the catalyzed signal amplification system we

SUBMITTER: Nishizuka S 

PROVIDER: S-EPMC283574 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature