Chimeric tRNAs as tools to induce proteome damage and identify components of stress responses.

ABSTRACT: Misfolded proteins are caused by genomic mutations, aberrant splicing events, translation errors or environmental factors. The accumulation of misfolded proteins is a phenomenon connected to several human disorders, and is managed by stress responses specific to the cellular compartments being affected. In wild-type cells these mechanisms of stress response can be experimentally induced by expressing recombinant misfolded proteins or by incubating cells with large concentrations of amino acid analogues. Here, we report a novel approach for the induction of stress responses to protein aggregation. Our method is based on engineered transfer RNAs that can be expressed in cells or tissues, where they actively integrate in the translation machinery causing general proteome substitutions. This s

SUBMITTER: Geslain R 

PROVIDER: S-EPMC2836549 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature