Chromosome arm length and nuclear constraints determine the dynamic relationship of yeast subtelomeres.

ABSTRACT: Physical interactions between distinct chromosomal genomic loci are important for genomic functions including recombination and gene expression, but the mechanisms by which these interactions occur remain obscure. Using telomeric association as a model system, we analyzed here the in vivo organization of chromosome ends of haploid yeast cells during interphase. We separately labeled most of the 32 subtelomeres and analyzed their positions both in nuclear space and relative to three representative reference subtelomeres by high-throughput 3D microscopy and image processing. We show that subtelomeres are positioned nonrandomly at the nuclear periphery, depending on the genomic size of their chromosome arm, centromere attachment to the microtubule organizing center (spindle pole body, SPB), a

SUBMITTER: Therizols P 

PROVIDER: S-EPMC2836701 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature