Genomic sequence of a mutant strain of Caenorhabditis elegans with an altered recombination pattern.

ABSTRACT:

Background

The original sequencing and annotation of the Caenorhabditis elegans genome along with recent advances in sequencing technology provide an exceptional opportunity for the genomic analysis of wild-type and mutant strains. Using the Illumina Genome Analyzer, we sequenced the entire genome of Rec-1, a strain that alters the distribution of meiotic crossovers without changing the overall frequency. Rec-1 was derived from ethylmethane sulfonate (EMS)-treated strains, one of which had a high level of transposable element mobility. Sequencing of this strain provides an opportunity to examine the consequences on the genome of altering the distribution of meiotic recombination events.

Results

Using Illumina sequencing and MAQ software, 83% of the base pair sequence reads w

SUBMITTER: Rose AM 

PROVIDER: S-EPMC2837035 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature