Analysis of particulate contaminations of infusion solutions in a pediatric intensive care unit.

ABSTRACT:

Purpose

To examine the physical properties and chemical composition of particles captured by in-line microfilters in critically ill children, and to investigate the inflammatory and cytotoxic effects of particles on endothelial cells (HUVEC) and macrophages in vitro.

Methods

Prospective, observational study of microfilters following their use in the pediatric intensive care unit. In vitro model utilizing cytokine assays to investigate the effects of particles on human endothelial cells and murine macrophages.

Results

Twenty filter membranes from nine patients and five controls were examined by electron microscopy (EM) and energy dispersion spectroscopy (EDX). The average number of particles found on the surface of the used membranes was 550 cm(2). EDX analysis confirm

SUBMITTER: Jack T 

PROVIDER: S-EPMC2837187 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature