Identification of the regulatory logic controlling Salmonella pathoadaptation by the SsrA-SsrB two-component system.

ABSTRACT: Sequence data from the past decade has laid bare the significance of horizontal gene transfer in creating genetic diversity in the bacterial world. Regulatory evolution, in which non-coding DNA is mutated to create new regulatory nodes, also contributes to this diversity to allow niche adaptation and the evolution of pathogenesis. To survive in the host environment, Salmonella enterica uses a type III secretion system and effector proteins, which are activated by the SsrA-SsrB two-component system in response to the host environment. To better understand the phenomenon of regulatory evolution in S. enterica, we defined the SsrB regulon and asked how this transcription factor interacts with the cis-regulatory region of target genes. Using ChIP-on-chip, cDNA hybridization, and comparative ge

SUBMITTER: Tomljenovic-Berube AM 

PROVIDER: S-EPMC2837388 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature