Heads or tails: L1 insertion-associated 5' homopolymeric sequences.

ABSTRACT:

Background

L1s are one of the most successful autonomous mobile elements in primate genomes. These elements comprise as much as 17% of primate genomes with the majority of insertions occurring via target primed reverse transcription (TPRT). Twin priming, a variant of TPRT, can result in unusual DNA sequence architecture. These insertions appear to be inverted, truncated L1s flanked by target site duplications.

Results

We report on loci with sequence architecture consistent with variants of the twin priming mechanism and introduce dual priming, a mechanism that could generate similar sequence characteristics. These insertions take the form of truncated L1s with hallmarks of classical TPRT insertions but having a poly(T) simple repeat at the 5' end of the insertion. We identif

SUBMITTER: Meyer TJ 

PROVIDER: S-EPMC2837659 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature