Comparative gene expression profiling between human cultured myotubes and skeletal muscle tissue.

ABSTRACT:

Background

A high-sensitivity DNA microarray platform requiring nanograms of RNA input facilitates the application of transcriptome analysis to individual skeletal muscle (SM) tissue samples. Culturing myotubes from SM-biopsies enables investigating transcriptional defects and assaying therapeutic strategies. This study compares the transcriptome of aneurally cultured human SM cells versus that of tissue biopsies.

Results

We used the Illumina expression BeadChips to determine the transcriptomic differences between tissue and cultured SM samples from five individuals. Changes in the expression of several genes were confirmed by QuantiGene Plex assay or reverse transcription real-time PCR. In cultured myotubes compared to the tissue, 1216 genes were regulated: 583 down and 633

SUBMITTER: Raymond F 

PROVIDER: S-EPMC2838843 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature