Validation of rearrangement break points identified by paired-end sequencing in natural populations of Drosophila melanogaster.

ABSTRACT: Several recent studies have focused on the evolution of recently duplicated genes in Drosophila. Currently, however, little is known about the evolutionary forces acting upon duplications that are segregating in natural populations. We used a high-throughput, paired-end sequencing platform (Illumina) to identify structural variants in a population sample of African D. melanogaster. Polymerase chain reaction and sequencing confirmation of duplications detected by multiple, independent paired-ends showed that paired-end sequencing reliably uncovered the break points of structural rearrangements and allowed us to identify a number of tandem duplications segregating within a natural population. Our confirmation experiments show that rates of confirmation are very high, even at modest coverage.

SUBMITTER: Cridland JM 

PROVIDER: S-EPMC2839345 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature