Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro.

ABSTRACT: The molecular oscillations underlying the generation of circadian rhythmicity in mammals develop gradually during ontogenesis. However, the developmental process of mammalian cellular circadian oscillator formation remains unknown. In differentiated somatic cells, the transcriptional-translational feedback loops (TTFL) consisting of clock genes elicit the molecular circadian oscillation. Using a bioluminescence imaging system to monitor clock gene expression, we show here that the circadian bioluminescence rhythm is not detected in the mouse embryonic stem (ES) cells, and that the ES cells likely lack TTFL regulation for clock gene expression. The circadian clock oscillation was induced during the differentiation culture of mouse ES cells without maternal factors. In addition, reprogrammin

SUBMITTER: Yagita K 

PROVIDER: S-EPMC2840478 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature