Soluble and mature amyloid fibrils in drusen deposits.

ABSTRACT:

Purpose

Drusen are a hallmark of eyes affected by age-related macular degeneration. In previous study, a conformational-specific antibody showed drusen to contain nonfibrillar amyloid structures. The current study was undertaken to assess the presence of additional amyloid structures in drusen.

Methods

Sections from human donor eyes were reacted with M204, a monoclonal antibody that recognizes nonfibrillar oligomers; OC, a polyclonal antibody that recognizes amyloid fibrils of various molecular weights; and WO1 and WO2, monoclonal antibodies that are specifically reactive to mature amyloid fibrils. Electron microscopy was used as an independent means of investigating the presence of amyloid fibrils in drusen.

Results

The presence of nonfibrillar oligomers was verified

SUBMITTER: Isas JM 

PROVIDER: S-EPMC2840723 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature