TAp73 is one of the genes responsible for the lack of response to chemotherapy depending on B-Raf mutational status.

ABSTRACT:

Background

Although there have been many studies on the p73 gene, some of its functions still remain unclear. There is little research on the relationship between p73 gene transcription and its protein expression and the response to certain drugs such as oxaliplatin and cetuximab, which are drugs currently used in colorectal cancer.The purpose of this study was to evaluate the impact of TAp73 expression on oxaliplatin and cetuximab-based chemotherapy in colorectal cancer cell lines with different K-Ras and B-Raf mutational status.

Methods

TAp73 was analyzed in three colorectal tumor cell lines HT-29, SW-480 and Caco-2. mRNA TAp73 was determined using Real time PCR; TAp73 protein by immunoblotting and cell viability was analyzed by the MTT method.

Results

We found that

SUBMITTER: Herreros-Villanueva M 

PROVIDER: S-EPMC2841128 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature