Selective regain of egfr gene copies in CD44+/CD24-/low breast cancer cellular model MDA-MB-468.

ABSTRACT:

Background

Increased transcription of oncogenes like the epidermal growth factor receptor (EGFR) is frequently caused by amplification of the whole gene or at least of regulatory sequences. Aim of this study was to pinpoint mechanistic parameters occurring during egfr copy number gains leading to a stable EGFR overexpression and high sensitivity to extracellular signalling. A deeper understanding of those marker events might improve early diagnosis of cancer in suspect lesions, early detection of cancer progression and the prediction of egfr targeted therapies.

Methods

The basal-like/stemness type breast cancer cell line subpopulation MDA-MB-468 CD44high/CD24-/low, carrying high egfr amplifications, was chosen as a model system in this study. Subclones of the heterogeneous c

SUBMITTER: Agelopoulos K 

PROVIDER: S-EPMC2841141 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature