Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.

ABSTRACT:

Background

Head and neck squamous cell carcinoma (HNSCC) is an aggressive and lethal malignancy. Publically available cell lines are mostly of lingual origin, or have not been carefully characterized. Detailed characterization of novel HNSCC cell lines is needed in order to provide researchers a concrete keystone on which to build their investigations.

Methods

The USC-HN1 cell line was established from a primary maxillary HNSCC biopsy explant in tissue culture. The immortalized cells were then further characterized by heterotransplantation in Nude mice; immunohistochemical staining for relevant HNSCC biomarkers; flow cytometry for surface markers; cytogenetic karyotypic analysis; human papillomavirus and Epstein-Barr virus screening; qRT-PCR for oncogene and cytokine analysi

SUBMITTER: Liebertz DJ 

PROVIDER: S-EPMC2841166 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature