Homer1a-dependent crosstalk between NMDA and metabotropic glutamate receptors in mouse neurons.

ABSTRACT:

Background

A large number of evidences suggest that group-I metabotropic glutamate receptors (mGluR1a, 1b, 1c, 5a, 5b) can modulate NMDA receptor activity. Interestingly, a physical link exists between these receptors through a Homer-Shank multi-protein scaffold that can be disrupted by the immediate early gene, Homer1a. Whether such a versatile link supports functional crosstalk between the receptors is unknown.

Methodology/principal findings

Here we used biochemical, electrophysiological and molecular biological approaches in cultured mouse cerebellar neurons to investigate this issue. We found that Homer1a or dominant negative Shank3 mutants that disrupt the physical link between the receptors allow inhibition of NMDA current by group-I mGluR agonist. This effect is antag

SUBMITTER: Bertaso F 

PROVIDER: S-EPMC2841198 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature